Nitric oxide inhibits the replication cycle of porcine parvovirus in vitro

作者：Wei Zhan Yong; Wang Xue Bin; Ning Xiao Dong; Wang Ya Bin; Zhang Hong Ying; Wang Dong Fang; Chen Hong Ying; Cui Bao An^*

来源：Archives of Virology, 2009, 154(6): 999-1003.

DOI：10.1007/s00705-009-0392-y

This study investigated the inhibitory effect and mechanism of nitric oxide (NO) on porcine parvovirus (PPV) replication in PK-15 cells. The results showed that two NO-generating compounds, S-nitroso-l-acetylpenicillamine (SNAP) and l-arginine (LA), at a noncytotoxic concentration could reduce PPV replication in a dose-dependent manner and that this anti-PPV effect could be reversed by the NO synthase (NOS) inhibitor N-nitro-l-arginine methyl ester (l-NAME). By assaying the steps of the PPV life cycle, we also show that NO inhibits viral DNA and protein synthesis. This experiment provides a frame of reference for the study of the anti-viral mechanism of NO.

• 出版日期2009-6
• 单位河南农业大学