Active inclusion body formation using Paenibacillus polymyxa PoxB as a fusion partner in Escherichia coli

作者：Park Soo Young; Park Seung Hwan; Choi Soo Keun^*

来源：Analytical Biochemistry, 2012, 426(1): 63-65.

DOI：10.1016/j.ab.2012.04.002

• Paenibacillus polymyxa
• PoxB
• Active inclusion body
• Recombinant protein expression

Overexpression of Paenibacillus polymyxa PoxB in Escherichia coli induced the formation of inclusion bodies. An enzyme assay showed that the inclusion bodies exhibited PoxB activity, indicating that they were biologically active. Fusion of GFP and Bacillus subtilis AmyE to the C-terminus of the PoxB also induced the formation of biologically active aggregates when they were overexpressed in E. coil. Therefore, P. polymyxa PoxB can be used as a fusion partner to promote the formation of active inclusion bodies in E. coli.

• 出版日期2012-7-1