Embryonic adhesion is not affected by endometrial leptin receptor gene silencing

作者：Cervero Ana; Dominguez Francisco; Horcajadas Jose Antonio; Quinonero Alicia; Pellicer Antonio; Simon Carlos^*

来源：Fertility and Sterility, 2007, 88: 1086-1092.

DOI：10.1016/j.fertnstert.2006.12.028

• embryonic adhesion
• implantation
• leptin receptor
• RNA interference

Objective: In rodents, evidence suggests that the leptin system is mandatory for embryonic implantation. We aimed to investigate the functional relevance of the endometrial leptin receptor (OB-R) in the adhesion phase of human implantation. Design: We used an in vitro model for embryonic adhesion, composed of a human endometrial cell line (HECl-A) and B6C3Fl mouse embryos. The OB-R gene was silenced in a stable manner by RNA interference, and embryonic adhesion rates were analyzed. Setting: Research laboratory at a university-affiliated center. Intervention(s): RNA interference. Main Outcome Measure(S): Embryonic adhesion in cells treated with OB-R RNAL Result(S): The OB-R shRNA-transfected cells exhibited up to 80% lower OB-R mRNA levels than those of cells nontransfected or transfected with scrambled shRNA. The OB-R protein was also highly diminished in the stable OB-R shRNA-transfected HEClA cells, whereas OASI expression was similar in both nontransfected and transfected cells. Embryonic adhesion rate was similar in nontransfected (94%) and HECl-A transfected cells with a scrambled sequence (94%) or with OB-R silencing sequence (92%). Conclusion(s): Knocking down the OB-R gene in a human endometrial cell line has no effect on the embryonic adhesion rate. Nevertheless, the functional relevance of this system can not be excluded in other phases of embryonic implantation, such as the invasion phase. Moreover, we describe a new approach to the functional analysis of candidate molecules implicated in blastocyst adhesion. (Fertil Steril (R) 2007;88(Suppl 2):1086-92.

• 出版日期2007-10