科研之友
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摘要
Hematopoietic stem cells (HSCs) are derived from endothelium in the aortic-gonadomesonephric (AGM) region during embryogenesis. But little is known about whether endothelial progenitor cells (EPCs) retain hematopoiesis potential after birth. In this study, we isolated adult EPCs from the bone marrow of C57BL/6 mice and identified them with an endothelial functional assay and by the CD31(+) CD133(+) CD45(-/dim) VEGFR2(+) phenotype. EPCs isolated from green fluorescence protein (GFP) transgenic C57BL/6 mice were cotransfused with bone marrow cells from wild-type C57BL/6 mice into lethally irradiated BABL/c mice. One month after transplantation, granulocytes (25.73 +/- 5.43%) and lymphocytes (12.68 +/- 3.26%) in peripheral blood showed GFP(+), referred to as donor EPC-derived blood cells. After an additional month, the percentage of GFP(+) granulocytes decreased to (3.69 +/- 1.43%), whereas the percentage of GFP(+) lymphocytes showed no significant difference. Most of the GFP(+) elements showed a diffuse distribution in the spleen; but some were present as aggregates forming lymphoid nodules. GFP(+) endothelial cells were observed in the liver sinusoids, intestinal villi, and lung of recipient mice. These results indicated that adult EPCs not only took part in vasculogenesis, but also retained hematopoetic ability.
