A sensitive flow injection chemiluminescence method for the determination of progesterone

作者：Cao, Wei^*; Gong, Pixue; Liu, Wenwen; Zhuang, Mei; Yang, Jinghe

来源：Drug Testing and Analysis, 2013, 5(4): 242-246.

DOI：10.1002/dta.299

• flow injection
• chemiluminescence
• progesterone
• potassium permanganate
• tris(1,10-phenanthroline)ruthenium(II)

A sensitive flow injection (FI) chemiluminescence (CL) method was developed for the determination of trace amounts of progesterone. This method was based on the luminescent properties of the tris(1,10-phenanthroline) ruthenium(II) - potassium permanganate (KMnO4) - progesterone in acidic medium sensitized by Na2SO3. With the peak height as a quantitative parameter applying optimum conditions, the relative CL intensity was linear with progesterone concentration in the range of 1.0 x 1010 approximate to 6.0 x 109 g center dot ml1 and 6.0 x 109 approximate to 4.0 x 108 g center dot ml1 with a detection limit of 7.1 x 1011 g center dot ml1. The relative standard deviation (RSD) was 2.79% for 1.0 x 108 g center dot ml1 progesterone (n = 11). The proposed method held low detection limit and was successfully applied to determination of progesterone in pharmaceutical preparations. The possible CL reaction mechanism was also discussed.

• 出版日期2013-4
• 单位山东大学; 济南大学