An RNA aptamer that specifically binds pancreatic adenocarcinoma up-regulated factor inhibits migration and growth of pancreatic cancer cells

作者：Kim Yun Hee; Sung Ho Jin; Kim Sukyoung; Kim Eun Ok; Lee Ji Won; Moon Ju Young; Choi Kyungho; Jung Ji Eun; Lee Yangsoon; Koh Sang Seok; Rhee Sue Goo; Heo Kyun^*; Kim In Hoo

来源：Cancer Letters, 2011, 313(1): 76-83.

DOI：10.1016/j.canlet.2011.08.027

• Aptamer
• SELEX
• PAUF
• Pancreatic cancer
• Anticancer drug

Previously, we reported that a novel secretory protein, pancreatic adenocarcinoma up-regulated factor (PAUF), which is highly expressed in pancreatic cancer and mediates the growth and metastasis of pancreatic cancer cells. In this study, we generated and characterized a 2'-fluoropyrimidine modified RNA aptamer (P12FR2) directed against human PAUF. P12FR2 binds specifically to human PAUF with an estimated apparent K(D) of 77 nM. P12FR2 aptamer inhibits PAUF-induced migration of PANC-1, human pancreatic cancer cells, in a wound healing assay. Moreover, intraperitoneal injection of P12FR2 decreased tumor growth by about 60% in an in vivo xenograft model with CFPAC-1 pancreatic cancer cells, without causing a loss of weight in the treated mice. Taken together, we propose here that PAUF-specific RNA aptamer, P12FR2, has the potential to be effective in the therapy of human pancreatic cancer.

• 出版日期2011-12-26