A recombinant TGF-beta 1 vaccine ameliorates diabetic nephropathy in OLETF rats

作者：Zhang, Haojun; Wang, Hua; Yan, Meihua; Zhao, Tingting; Lu, Xiaoguang; Zhu, Bin; Gong, Yuewen^*; Li, Ping^*

来源：Immunotherapy, 2016, 8(9): 1045-1057.

DOI：10.2217/imt-2015-0005

• diabetic nephropathy
• OLETF rats
• transforming growth factor beta 1
• vaccine

Aim: The aim of this study was to investigate the potential of a recombinant vaccine encoding TGF-beta 1 in OLETF rats with diabetic nephropathy (DN). Methods: OLETF rats were treated with vehicle or TGF-beta 1 vaccine. LETO rats were used as normal controls. At 42 weeks after immunization with vaccine, samples from blood, urine and kidney were collected for biochemical, histologic, immunohistochemical and molecular analyses. Results: OLETF rats treated with the vaccine reduced blood glucose levels, improved renal pathological changes, and inhibited overexpression of TGF-beta 1 and p-Smad3, as well as MCP-1, TNF-alpha and IL-1 beta. Conclusion: TGF-beta 1 vaccine attenuated diabetic nephropathy in OLETF rats through reduction of inflammation, improvement of kidney fibrosis and partial correction of glucose metabolism.

• 出版日期2016-9
• 单位中国中医科学院; 中国中医科学院广安门医院; 中日友好医院