A mecA-Negative Strain of Methicillin-Resistant Staphylococcus aureus with High-Level beta-Lactam Resistance Contains Mutations in Three Genes

作者：Banerjee Ritu^*; Gretes Michael; Harlem Christopher; Basuino Li; Chambers Henry F

来源：Antimicrobial Agents and Chemotherapy, 2010, 54(11): 4900-4902.

DOI：10.1128/AAC.00594-10

We previously generated a ceftobiprole-resistant Staphylococcus aureus strain after high inoculum serial passage of a mecA-negative variant of strain COL (R. Banerjee, M. Gretes, L. Basuino, N. Strynadka, and H. F. Chambers, Antimicrob. Agents Chemother. 52:2089-2096, 2008). Genome resequencing of this strain, CRB, revealed that it differs from its parent by five single-nucleotide polymorphisms in three genes, specifically, those encoding PBP4, a low-molecular-weight penicillin-binding protein, GdpP, a predicted signaling protein, and AcrB, a cation multidrug efflux transporter. CRB displayed resistance to a variety of beta-lactams but was hypersusceptible to cefoxitin.

• 出版日期2010-11