An Application of Outer Membrane Protein P6-Specific Enzyme-Linked Immunosorbent Assay for Detection of Haemophilus influenzae in Middle Ear Fluids and Nasopharyngeal Secretions

作者：Hotomi Muneki^*; Togawa Akihisa; Kono Masamitsu; Sugita Gen; Sugita Rinya; Fujimaki Yutaka; Kamide Yosuke; Uchizono Akihiro; Kanesada Keiko; Sawada Shoichi; Okitsu Naohiro; Masuda Hisayo; Tanaka Hideaki; Tanaka Yumi; Yamanaka Noboru

来源：PLos One, 2013, 8(8): e71774.

DOI：10.1371/journal.pone.0071774

An enzyme-linked immunosorbent assay specific to outer membrane protein P6 (P6-ELISA) was applied for detecting Haemophilus influenzae in middle ear fluids (MEFs) from acute otitis media (AOM) patients and in nasopharyngeal secretions (NPSs) from acute rhinosinusitis patients. P6-ELISA had a sensitivity of 83.3% for MEFs and 71.5% for NPSs and a specificity of 85.6% for MEFs and 92.5% for NPSs, respectively. Real-time PCR exhibited significant differences in the number of ompP1 gene copies among samples determined by P6-ELISA to be positive and negative for H. influenzae. However, because the P6-ELISA test has the reactivity in Haemophilus species include two commensals H. haemolyticus and H. parainfluenzae, it is thus a weak method in order to detect only NTHi correctly. Consequently, diagnosis using the P6-ELISA should be based on an overall evaluation, including the results of other related examinations and clinical symptoms to prevent misleading conclusions in clinical setting.

• 出版日期2013-8-28