Determination of the hydrolysis rate constants and activation energy of aesculin with capillary electrophoresis end-column amperometric detection

作者：Zhang L; Tong P; Chen GN^*

来源：Journal of Chromatography A, 2005, 1098(1-2): 194-198.

DOI：10.1016/j.chroma.2005.10.029

• capillary electrophoresis
• amperometric detection
• hydrolysis rate constant
• activation energy
• aesculin
• aesculetin

Aesculetin is the product of the hydrolysis reaction of aesculin. A high sensitivity and good repeatability method based on capillary electrophoresis with amperometric detection (CE-AD) was developed for simultaneous determination of aesculin and aesculetin in the hydrolysate of aesculin. Under the optimum condition: 10 mmol/L KH2PO4-5 mmol/L Na2B4O7 (pH 6.0) buffer, separation at 18 kV and +900 mV (versus Ag/AgCl) as the detection potential, the hydrolysis rate constants of aesculin hydrolysis at 25, 30, 35 40 and 45 C in 0.1 mol/L KOH were obtained as 1.45 x 10(-2) min(-1), 2.01 x 10(-2) min(-1), 2.93 x 10(-2) min(-1), 3.76 x 10(-2) min(-1) and 5.05x 10(-2) min(-1) respectively. It was calculated that the activation energy for aesculin hydrolysis was 49.4 kJ/mol.

• 出版日期2005-12-9
• 单位福州大学