First Trimester Screening of Circulating C19MC microRNAs Can Predict Subsequent Onset of Gestational Hypertension

作者：Hromadnikova Ilona^*; Kotlabova Katerina; Hympanova Lucie; Doucha Jindrich; Krofta Ladislav

来源：PLos One, 2014, 9(12): e113735.

DOI：10.1371/journal.pone.0113735

• Animals
• Base Sequence
• Caenorhabditis elegans
• Chromosomes
• Human
• Pair 19
• Female
• Humans
• Hypertension
• Pregnancy-Induced
• MicroRNAs
• Placenta
• Pregnancy
• Pregnancy Trimester
• First
• Prospective Studies

Objective: The objective of the study was to evaluate risk assessment for gestational hypertension based on the profile of circulating placental specific C19MC microRNAs in early pregnancy. %26lt;br%26gt;Study design: The prospective longitudinal cohort study of women enrolled at first trimester screening at 10 to 13 weeks was carried out (n=267). Relative quantification of placental specific C19MC microRNAs (miR-516-5p, miR-517*, miR-518b, miR-520a*, miR-520h, miR-525 and miR-526a) was determined in 28 normal pregnancies and 18 pregnancies which developed gestational hypertension using real-time PCR and a comparative Ct method relative to synthetic C. elegans microRNA (cel-miR-39). %26lt;br%26gt;Results: Increased extracellular C19MC microRNA plasmatic levels (miR-516-5p, p%26lt;0.001; miR-517*, p-0.007; miR-520h, p%26lt;0.001; miR-518b, p=0.002) were detected in patients destined to develop gestational hypertension. MiR-520h had the best predictive performance with a PPV of 84.6% at a 7.1% false positive rate. The combination of miR-520h and miR-518b was able to predict 82.6% of women at the same false positive rate. The overall predictive capacity of single miR-518b (73.3% at 14.3% FPR), miR-516-5p (70.6% at 17.9% FPR) and miR-517* (57.9% at 28.6% FPR) biomarkers was lower. %26lt;br%26gt;Conclusion: The study brought interesting finding that the up-regulation of miR516-5p, miR-517*, miR-520h and miR-518b is associated with a risk of later development of gestational hypertension. First trimester screening of extracellular miR-520h alone or in combination with miR-518b identified a significant proportion of women with subsequent gestational hypertension.

• 出版日期2014-12-15