Association of age with sperm DNA fragmentation

作者：Horta Fabrizzio; Madariaga Marcia; Garcia Alejandra; Hartel Steffen; Smith Rosita^*

来源：Revista Medica de Chile, 2011, 139(3): 306-312.

DOI：10.4067/S0034-98872011000300004

• Aging
• DNA fragmentation
• Spermatozoa

Background: There is an association between aging and an increased number of sperms with alterations in nuclear DNA. Aim: To study the association between age and fragmentation of sperm DNA. Material and Methods: Sixty two volunteers provided semen for analysis. These were separated in a group aged less than forty years and a second group aged more than forty years. Sperm DNA fragmentation was studied by TUNEL (terminal deoxynucleotidyl transferase-mediated 2'-deoxyuridine 5'-triphosphate nick end-labeling) and SCD (sperm chromatin dispersion test) assays. Results: Compared with their younger counterparts, patients aged more than 40 years had a higher proportion of sperms with DNA fragmentation by TUNEL (20 +/- 1.3 and 24 +/- 1.9% respectively, p < 0.05) and SCD (22 +/- 1.4 and 26 +/- 1.6% respectively, p < 0.05). The results of both assays had a correlation coefficient of 0.8. No differences between groups were observed for other seminal parameters. Conclusions: Sperm DNA fragmentation increases with age in males. (Rev Med Chile 2011; 139: 306-312).

• 出版日期2011-3