A comparison of the production efficiencies of full-length and truncated forms of prochymosin

作者：Badiefar Leila; Khodabandeh Mahvash^*; Ahmadian Gholamreza

来源：International Journal of Dairy Technology, 2011, 64(2): 288-293.

DOI：10.1111/j.1471-0307.2010.00664.x

• Prochymosin
• Recombinant protein
• Optimisation
• Fermentation

Chymosin is one of the enzymes with many applications in the food industry. Its recombinant type has been designed and constructed in different forms. In this investigation, the production efficiency of two types of complete and exon-6 less prochymosin was assessed. It was found that the average efficiencies of the optimum growth conditions calculated for the two constructs harbouring pET-prochymosin and pET-exon-6 less prochymosin were significantly different (P value < 0.05) at 192.02 +/- 3.57 and 348.87 +/- 7.76, respectively. Also during fermentation, the level of the exon-6 less prochymosin production was much higher than the other construct as demonstrated by SDS-PAGE.

• 出版日期2011-5