The Apyrase gene of the vector mosquito, Aedes aegypti, is expressed specifically in the adult female salivary glands

作者：Smartt CT^*; Kim AP; Grossman GL; James AA

来源：Experimental Parasitology, 1995, 81(3): 239-248.

DOI：10.1006/expr.1995.1114

• apyrase
• Aedes aegypti
• mosquito
• gene sequence
• salivary gland
• ADP, adenosine diphosphate
• AMP, adenosine monophosphate
• ATP, adenosine triphosphate
• cDNA, ''copy'' DNA
• SDS, sodium dodecyl sulfate

The yellow fever mosquito, Aedes aegypti, expresses a gene, Apyrase (Apy), that encodes an ATP-diphosphohydrolase The product of this gene is a secreted enzyme that facilitates hematophagy by preventing platelet aggregation in the host. Apy gene expression is limited to the cells of the distal-lateral and medial lobes of the adult female salivary glands. Apyrase protein levels, detectable by antibodies, peak in the salivary glands about 4 days after adult emergence and remain high after a blood meal. Primary sequence analysis of a genomic clone encoding apyrase reveals a unique TAAATA sequence and seven introns, as well as other conserved features of eukaryotic genes. The temporal, sex- and tissue-specific expression of the Apy gene is consistent with its role as encoding a platelet anti-aggregation factor that functions to facilitate hematophagy and decrease probing time.

• 出版日期1995-11