Dyeing and antibacterial activation with methylene blue of a cyclodextrin modified polyester vascular graft

作者：Kacem I; Laurent T; Blanchemain N^*; Neut C; Chai F; Haulon S; Hildebrand H F; Martel B

来源：Journal of Biomedical Materials Research Part A, 2014, 102(9): 2942-2951.

DOI：10.1002/jbm.a.34965

• cyclodextrin
• methylene blue
• vascular prosthesis
• adsorption isotherm
• antibacterial activity

The aim of this study was to develop an antiseptic and blue dyed polyester (PET) vascular graft in order to reach two distinct properties: (i) the prevention of postoperative infections, (ii) the improvement of the graft compatibility with the coelioscopy surgical technique. This work consisted of dyeing a vascular prosthesis with methylene blue (MB) which is known as a cationic dye with antiseptic properties. Therefore, the functionalization of the PET fibers of the prosthesis with a cyclodextrin-citric acid polymer (PolyCD) was achieved in order to improve its sorption capacity. The NMR experiments demonstrated that a 1: 2 complex occurred between hydroxypropyl beta-cyclodextrin (HP-bCD) and MB. Kinetic and sorption isotherm studies showed that an impregnation of the polyCD modified prosthesis (PET-CD) in a 1 g L-1 of MB solution for 150 min was sufficient to reach the saturation of the device. Results proved that the adsorption mechanism followed the Langmuir model and a maximum of 20 mg g(-1) of MB on the graft. A sustained release of MB in batch tests was observed in PBS and in vitro microbiological assays displayed a prolongation of the bactericidal effect of PET-CD whose extent varied with the amount of MB preliminarily adsorbed onto the PET-CD.

• 出版日期2014-9