Size-Exclusive Magnetic Graphene/Mesoporous Silica Composites with Titanium(IV)-Immobilized Pore Walls for Selective Enrichment of Endogenous Phosphorylated Peptides

作者：Sun Nianrong; Deng Chunhui^*; Li Yan; Zhang Xiangmin

来源：ACS Applied Materials & Interfaces, 2014, 6(14): 11799-11804.

DOI：10.1021/am502529a

• magnetic graphene
• mesoporous silica
• size-exclusive
• titanium
• endogenous phosphorylated peptides

Developing an effective separation method is necessary for identifying low-abundant endogenous phosphorylated peptides with the removal of proteins. In this work, we prepared size-exclusive magnetic graphene/mesoporous silica composites with titanium(lV)-immobilized pore walls (denoted as Ti4+-MGMSs) for capturing endogenous phosphorylated peptides for mass spectrometry analysis. The introduction of hydrophilic polydopamine simplified the synthetic process of Ti4+-MGMSs, and the ordered mesoporous channels are beneficial to the trapping of endogenous phosphopeptides while large-size proteins are excluded. Furthermore, the magnetic performance greatly simplifies the entire process of enrichment. With all of the advances, the novel Ti4+-MGMSs present high enrichment efficiency either from the low concentration of beta-casein tryptic digest (0.5 fmol/mu L) or the mixture of beta-casein tryptic digest and alpha-casein (or plus bovine serum albumin, with a mass ratio up to 1:500). Besides, Ti4+-MGMSs have also been successfully applied to enrich endogenous phosphorylated peptides from human serum and human saliva.

• 出版日期2014-7-23
• 单位复旦大学