A mediatorless and label-free amperometric immunosensor for detection of h-IgG

作者：Zhang Lingyan; Liu Yan^*; Chen Tao

来源：International Journal of Biological Macromolecules, 2008, 43(2): 165-169.

DOI：10.1016/j.ijbiomac.2008.04.010

• mediatorless
• label-free
• amperometric immunosensor

A novel experimental methodology for studying a mediatorless and label-free immunosensor is proposed by immobilizing antibody on gold nanoparticle/L-cysteine coated electrode (nano-Au/L-cysteine electrode). Differential pulse voltammograms (DPV) resulting from the assembled immunosensor indicate that the immunosensor shows excellent electrochemical response to dopamine so that the electrochemical response is utilized for the signal generation step of the immunosensor. Therefore, by means of unenzymatic-labeling procedure combined with the amperometric detection using dopamine as substrate, the immunological reaction can be detected. After the immunosensor is incubated with h-IgG solution, the access of electrocatalytic behavior center of the immunosensor to dopamine is partly inhibited, which leads to a linear decrease in amperometric response of the immunosensor with h-IgG concentration over a range 0.82-90 ng mL(-1) by DPV.

• 出版日期2008-8-15
• 单位重庆师范大学; 西南政法大学