Structure of the mammalian oligosaccharyltransferase complex in the native ER protein translocon

作者：Pfeffer Stefan; Dudek Johanna; Gogala Marko; Schorr Stefan; Linxweiler Johannes; Lang Sven; Becker Thomas; Beckmann Roland; Zimmermann Richard; Foerster Friedrich

来源：Nature Communications, 2014, 5(1): 3072.

DOI：10.1038/ncomms4072

In mammalian cells, proteins are typically translocated across the endoplasmic reticulum (ER) membrane in a co-translational mode by the ER protein translocon, comprising the protein-conducting channel Sec61 and additional complexes involved in nascent chain processing and translocation. As an integral component of the translocon, the oligosaccharyl-transferase complex (OST) catalyses co-translational N-glycosylation, one of the most common protein modifications in eukaryotic cells. Here we use cryoelectron tomography, cryoelectron microscopy single-particle analysis and small interfering RNA-mediated gene silencing to determine the overall structure, oligomeric state and position of OST in the native ER protein translocon of mammalian cells in unprecedented detail. The observed positioning of OST in close proximity to Sec61 provides a basis for understanding how protein translocation into the ER and glycosylation of nascent proteins are structurally coupled. The overall spatial organization of the native translocon, as determined here, serves as a reliable framework for further hypothesis-driven studies.

• 出版日期2014-1