Aluminum-mediated metabolic changes in rat serum and urine: A proton nuclear magnetic resonance study

作者：Tripathi Sandeep; Somashekar B S; Mahdi Abbas Ali; Gupta Ashish; Mahdi Farzana; Hasan Mahdi; Roy Raja; Khetrapal C L^*

来源：Journal of Biochemical and Molecular Toxicology, 2008, 22(2): 119-127.

DOI：10.1002/jbt.20219

• Al3+ toxicity
• H-1 NMR
• rat serum
• rat urine
• metabolic alterations
• TCA cycle

The toxic effects of Al3+ have been studied in 90-days AlCl3 orally treated male albino rats (n=7) using H-1 NMR spectroscopy-based metabolic profile of rat serum and urine, serum enzyme tests, behavioral impairment, and histopathology of kidney and liver. Metabolic profile of 90-days Al3+-treated rat sera showed significantly elevated levels of alanine, glutamine, beta-hydroxy-butyrate, and acetoacetate and significantly decreased level of acetone when compared with that of control rats. However, metabolic profile of 90-days Al3+-treated rat urine showed significantly decreased levels of citrate, creatinine, allantoin, trans-aconitate, and succinate and significantly increased level of acetate when compared to control rats. The overall perturbations observed in the metabolic profile of serum and urine demonstrate the impairment in the tricarboxylic acid cycle, liver and kidney metabolism, which was further reinstated by clinical chemistry and histopathological observations. Moreover, "in vivo" behavioral impairment has also been observed as the indication of aluminum neurotoxicity.

• 出版日期2008
• 单位河北医科大学