Uninduced high-yield bacterial expression of fluorescent proteins

作者：Sarabipour Sarvenaz; King Christopher; Hristova Kalina^*

来源：Analytical Biochemistry, 2014, 449: 155-157.

DOI：10.1016/j.ab.2013.12.027

• Fluorescent protein
• Gene expression
• Protein production
• High yield
• FRET
• His-tag
• E. coli

Here we introduce a fast, cost-effective, and highly efficient method for production of soluble fluorescent proteins from bacteria. The method does not require optimization and does not use isopropyl beta-D-1-thiogalactopyranoside (IPTG) induction. The method relies on uninduced expression in the BL21-Gold (DE3) strain of Escherichia coli and yields large amounts (up to 0.4 mu mol) of fluorescent protein from a 250-ml culture. This method is much simpler than published methods and can be used to produce any fluorescent protein that is needed in biomedical research.

• 出版日期2014-3-15