An azide functionalized oligothiophene ligand - A versatile tool for multimodal detection of disease associated protein aggregates

作者：Johansson Leif B G; Simon Rozalyn; Bergstrom Gunnar; Eriksson Mikaela; Prokop Stefan; Mandenius Carl Fredrik; Heppner Frank L; Aslund Andreas K O; Nilsson K Peter R^*

来源：Biosensors and Bioelectronics, 2015, 63: 204-211.

DOI：10.1016/j.bios.2014.07.042

• Protein aggregates
• Oligothiophene
• Fluorescence
• Surface plasmon resonance
• Click chemistry

Ligands for identifying protein aggregates are of great interest as such deposits are the pathological hallmark of a wide range of severe diseases including Alzheimer's and Parkinson's disease. Here we report the synthesis of an azide functionalized fluorescent pentameric oligothiophene that can be utilized as a ligand for multimodal detection of disease-associated protein aggregates. The azide functionalization allows for attachment of the ligand to a surface by conventional click chemistry without disturbing selective interaction with protein aggregates and the oligothiophene-aggregate interaction can be detected by fluorescence or surface plasmon resonance. In addition, a methodology where the oligothiophene ligand is employed as a capturing molecule selective for aggregated proteins in combination with an antibody detecting a distinct peptide/protein is also presented. We foresee that this methodology will offer the possibility to create a variety of multiplex sensing systems for sensitive and selective detection of protein aggregates, the pathological hallmarks of several neurodegenerative diseases.

• 出版日期2015-1-15