Differential cellular and microbial responses to nano-/micron-scale titanium surface roughness induced by hydrogen peroxide treatment

作者：Daw Abdulaziz E; Kazi Hizbullah A A; Colombo John S; Rowe Wendy G; Williams David W; Waddington Rachel J; Thomas David W; Moseley Ryan^*

来源：Journal of Biomaterials Applications, 2013, 28(1): 144-160.

DOI：10.1177/0885328212441495

• Bone marrow stromal cell
• hydrogen peroxide
• osseointegration
• osteoblast
• peri-implantitis
• surface roughness
• titanium

This study investigated the effects of combined titanium nano-/micron-scale roughness, induced by hydrogen peroxide pre-treatments, on bone marrow stromal cell responses and Porphyromonas gingivalis adherence in vitro. Untreated surfaces exhibited nano-scale features, while hydrogen peroxide treatments promoted increased nano-/micron-scale roughness. Bone marrow stromal cell attachment and proliferation were maintained with 6 h and 24 h treatments, but significantly decreased on 1-week and 4-week-treated surfaces. Bone marrow stromal cells on 6 h-4 week-treated titanium demonstrated enhanced osteogenic differentiation versus untreated surfaces. P. gingivalis adherence was significantly increased on 24 h-4 week surfaces. Results suggest that 6 h but less than 24 h treatments maintain or promote bone marrow stromal cell responses while minimizing microbial adherence, potentially enhancing titanium surface bio-activation for osseointegration.

• 出版日期2013-7