The influence of diet on intra and inter-individual variability of urinary excretion of arsenic species in Italian healthy individuals

作者：Lovreglio Piero^*; D' Errico Maria Nicola; Gilberti Maria Enrica; Drago Ignazio; Basso Antonella; Apostoli Pietro; Soleo Leonardo

来源：Chemosphere, 2012, 86(9): 898-905.

DOI：10.1016/j.chemosphere.2011.10.050

• Arsenic
• Inter-individual variability
• Intra-individual variability
• Urinary speciation
• Seafood
• Fish

To study the effect of eating foods with a high arsenic (As) content on the intra and inter-individual variability of urinary concentrations of the As species, daily urine samples were collected for 10 consecutive days from 12 healthy male subjects. A daily food diary was kept throughout the study period. Personal exposure to airborne As was measured once during the study. As-3, As-5, monomethylarsonic acid (MMA), dimethylarsinic acid (DMA) and arsenobetaine were determined in all urine samples by inductive coupled plasma mass spectrometry, and the sum of As-3 + As-5 + MMA + DMA (iAs) by hydride generation-atomic absorption spectrophotometry. Exposure to airborne As was below the limit of detection in all samplings. As-3 was found in only 19.2% and Ass in only 3.3% of the urine samples, whereas high urinary concentrations of arsenobetaine were observed. With the exception of arsenobetaine, expressed as a percentage, a significant inter-individual variability was observed for all species of As, for iAs and for the MMA/DMA ratio (p %26lt; 0.001). Instead, the intra-individual variability was significant only for the MMA/DMA ratio (p %26lt; 0.001). Among foods with a high As content, only a heavy consumption of seafood was shown to influence inter-individual variability of DMA%, arsenobetaine expressed as mu g g(-1) creatinine and iAs. In conclusion, even in populations with a high intake of organic As through foods, the finding of a significant inter-individual but no significant intra-individual variability of urinary species confirms the usefulness of urinary As speciation for biological monitoring of exposure to As.

• 出版日期2012-3