Accumulation of flavonoids and antioxidant activity of Stellera chamaejasme by an efficient callus culture

作者：Wang Junli^*; Xiao Xuan; Wang Qian; Li Xiaoxu; Zhang Lu; Li Jianfei

来源：Horticulture Environment and Biotechnology, 2013, 54(5): 441-449.

DOI：10.1007/s13580-013-0171-2

• callus proliferation
• plant growth regulators
• radical scavenging activity

An efficient callus proliferation system of Stellera chamaejasme was developed. The calli were initially induced by cultivating the leaf explants on the MS medium containing 1.0 mg center dot L-1 2,4-dichlorophenoxy acetic acid (2,4-D). The culture had its fresh and dry weights increased by about 29 and 25 times, respectively, through further cultivation on the MS medium containing 0.5 mg center dot L-1-naphthalene acetic acid (NAA) and 1.0 mg center dot L-1 n-phenyl-n' -1,2,3-thiadiazol-5-ylurea (TDZ). The concentrations of NAA and 6-benzylaminopurine (BA) for an efficient accumulation of the total flavonoids in the callus were found to be 1.0 mg center dot L-1 and 0.25 mg center dot L-1, respectively. With this combination, the content of the total flavonoids slightly increased to 10.8 mg center dot g(-1) dry weight (DW) in comparison to 10.1 mg center dot g(-1) DW obtained in the root of wild-type plant. The antioxidant activities of all flavonoid extracts were evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The flavonoid extracts from the callus as induced by 1.0 mg center dot L-1 NAA and 0.25 or 0.5 mg center dot L/t-1 BA was very active in radical scavenging, and their IC50 values were 11.94 and 19.17 g center dot mL(-1), respectively. Compared to the ascorbic acid (IC50 21.21 g center dot mL(-1)), the antioxidant activity of callus from S. chamaejasme was even stronger, suggesting that be another potential source of new natural antioxidants.

• 出版日期2013-10
• 单位中央民族大学