Array-based sensing of purine derivatives with fluorescent dyes

作者：Koestereli Ziya; Severin Kay^*

来源：Organic and Biomolecular Chemistry, 2015, 13(35): 9231-9235.

DOI：10.1039/c5ob01201e

Natural and synthetic purine derivatives such as caffeine, theophylline, 6-mercaptopurine and 8-chloro-theophylline are important drugs. Due to the structural similarity of these compounds, it is intrinsically difficult to prepare chemosensors for their selective optical detection. Here, we describe a sensor array which can be used to differentiate pharmacologically important purine derivatives with good accuracy. The array is composed of four polysufonated fluorescent dyes, all of which can bind purines via pi-stacking interactions. The complexation of the analytes results in partial quenching of the fluorescence. The fluorescence response of the four dyes provides a characteristic signal pattern, enabling the identification of thirteen purine derivatives at low millimolar concentration. Furthermore, it is possible to use the array for obtaining information about the quantity and purity of purine samples.

• 出版日期2015