Analysis of l-tyrosine based on electrocatalytic oxidative reactions via screen-printed electrodes modified with multi-walled carbon nanotubes and nanosized titanium oxide (TiO2)

作者：Shumyantseva Victoria^*; Bulko Tatiana; Kuzikov Alexey; Masamrekh Rami; Archakov Alexander

来源：Amino Acids, 2018, 50(7): 823-829.

DOI：10.1007/s00726-018-2557-z

• l-Tyrosine
• Electrocatalytic oxidation
• TiO2
• Protein electrochemistry
• Multi-walled carbon nanotubes
• Modified electrodes

Method for electrochemical determination of l-tyrosine with screen-printed electrodes (SPE) modified with multi-walled CNT or CNT/TiO2 as sensing elements was used for the electroanalysis of l-tyrosine (Tyr). It was demonstrated that SPE/CNT and SPE/CNT/TiO2 exhibited high electrocatalytic activity and good analytical performance towards oxidation of l-tyrosine. The linear range of Tyr in human serum was 0.025 A center dot 1 mM with the correlation coefficient R (2) = 0.97. Direct electrochemistry (without any mediator) of co-factor-free bovine serum albumin (BSA) and human serum albumin (HSA) was investigated by use of modified electrodes. Protein-ligand interactions based on the electrocatalytic oxidation of l-tyrosine during HSA interaction with hemin were analyzed by the change of peak height and oxidation peak area, corresponding to tyrosine oxidation accessibility.

• 出版日期2018-7