Molecularly imprinted polymers for the recognition of sodium dodecyl sulfate denatured creatine kinase

作者：Wang Chung Yih^*; Chen Yi Chen; Sheu Dey Chyi; Chou Tse Chuan

来源：Journal of the Taiwan Institute of Chemical Engineers, 2012, 43(2): 188-194.

DOI：10.1016/j.jtice.2011.10.001

• Molecularly imprinted polymer
• Creatine kinase
• Protein recognition

Micro-contact imprinting methodology was used to prepare the molecule recognition film for denatured creatine kinase-MM (CK-MM), which was denatured by sodium dodecyl sulfate. Interpretation of thermo-calorimetry measurements suggested the use of poly(ethylene glycol) 400 dimethacrylate (PEG400DMA) as a crosslinking molecule. The selected functional monomer, methyl methacrylate (MMA), produced the highest imprinting factor from a panel of five candidates. The molecularly imprinted polymers (MIPs) formed with 5% MMA, 95% PEG400DMA and denatured OK-MM showed excellent imprint recognition, with the imprinting factor of 8.66. The dissociation constant calculated from Scatchard plot was 3.25 x 10(-8) M. MIPs had little affinity with non-template proteins, such as native CK-MM, a native and denatured form of human serum albumin (HSA) and immunoglobulin G (IgG). The competitive study in a two-protein environment showed that the selectivity of MIP was 96.8% and 98.7% for denatured CK-MM/denatured HSA and denatured CK-MM/denatured IgG, respectively. The results suggested that MIPs prepared in this work recognize not only the protein sequences but also the protein secondary structure.

• 出版日期2012-3