A nanocomposite prepared from FeOOH and N-doped carbon nanosheets as a peroxidase mimic, and its application to enzymatic sensing of glucose in human urine

作者：Tran, Hoang V.^*; Nguyen, Tuan V.; Nguyen, Nghia D.; Piro, Benoit; Huynh, Chinh D.

来源：Microchimica Acta, 2018, 185(5): 270.

DOI：10.1007/s00604-018-2804-8

• Enzyme-lesshydrogenperoxidedetection
• Glucoseassay
• Colorimetric assay,3,3',5,5'-tetramethylbenzidine(TMB)
• Urine glucose
• Glucose oxidase
• Diabetes
• Bare-eye assay
• Peroxidase mimetic catalyst

A method is described for the synthesis of a nanocomposite containing FeOOH and N-doped carbon nanosheets. The nanocomposite was synthesized by a hydrothermal method using a Fe3O4/chitosan nanocomposite as the precursor. The nanocomposite displays peroxidase-like activity and catalyzes the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) by H2O2. This results in the formation of a blue colored product with an absorption maximum at 652 nm in the UV-vis spectra. Based on these findings, colorimetric assays were worked out for both hydrogen peroxide and glucose. The H2O2 assay works in the 5 to 19 mu M concentration range, and the limit of detection is 5 nM. The glucose assay works in the 8 mu M to 0.8 mM concentration range and has a 0.2 mu M detection limit. The method was successfully applied to the determination of glucose in human urine.

• 出版日期2018-5
• 单位华中科技大学