Digital cell quantification identifies global immune cell dynamics during influenza infection

作者：Altboum Zeev; Steuerman Yael; David Eyal; Barnett Itzhaki Zohar; Valadarsky Liran; Keren Shaul Hadas; Meningher Tal; Mendelson Ella; Mandelboim Michal^*; Gat Viks Irit; Amit Ido

来源：Molecular Systems Biology, 2014, 10(2): 720-720.

DOI：10.1002/msb.134947

• dendritic cells
• cell quantification
• immune cell dynamics
• influenza infection
• deconvolution approach

Abstract %26lt;br%26gt;Hundreds of immune cell types work in coordination to maintain tissue homeostasis. Upon infection, dramatic changes occur with the localization, migration, and proliferation of the immune cells to first alert the body of the danger, confine it to limit spreading, and finally extinguish the threat and bring the tissue back to homeostasis. Since current technologies can follow the dynamics of only a limited number of cell types, we have yet to grasp the full complexity of global in vivo cell dynamics in normal developmental processes and disease. Here, we devise a computational method, digital cell quantification (DCQ), which combines genome-wide gene expression data with an immune cell compendium to infer in vivo changes in the quantities of 213 immune cell subpopulations. DCQ was applied to study global immune cell dynamics in mice lungs at ten time points during 7 days of flu infection. We find dramatic changes in quantities of 70 immune cell types, including various innate, adaptive, and progenitor immune cells. We focus on the previously unreported dynamics of four immune dendritic cell subtypes and suggest a specific role for CD103(+) CD11b(-) DCs in early stages of disease and CD8(+) pDC in late stages of flu infection. %26lt;br%26gt;Synopsis %26lt;br%26gt;image %26lt;br%26gt;A method is presented to infer the changes in the quantities of 213 immune cell types within a complex in vivo cell population. High-resolution temporal analysis during flu infection reveals specific roles of dendritic cell subtypes in early and late disease phases. %26lt;br%26gt;A systematic approach for exploring in vivo immune cell dynamics is presented. %26lt;br%26gt;Computational quantification of over 200 immune cell subpopulations is possible. %26lt;br%26gt;A comprehensive view of influenza infection dynamics uncovers changes in dozens of distinct immune cell subpopulations. %26lt;br%26gt;Plasmacytoid dendritic cells serve as a cavalry to maintain long-lasting host defense against influenza infection.

• 出版日期2014-2