A REPETITIVE SEQUENCE IN NICOTIANA-PLUMBAGINIFOLIA CONTAINS AN ELEMENT HOMOLOGOUS TO THE HYPOTHETICAL REPLICATION ORIGINS OF PLANT-VIRUSES

作者：CHEN CM^*; LEE LC

来源：Botanical Bulletin of Academia Sinica, 1993, 34(4): 313-322.

• AUTONOMOUSLY REPLICATING SEQUENCE
• DNA-BINDING NUCLEAR FACTOR
• NICOTIANA-PLUM-BAGINIFOLIA
• REPLICATION ORIGIN
• TANDEM REPEATED SEQUENCE

Twelve genomic DNA fragments displaying autonomous replicative activity in yeast have been cloned from Nicotiana plumbaginifolia. One of them interacted specifically with nuclear factor(s) from young leaves of this plant, at a region containing 9 copies of tandem repeated sequence. This repeated sequence appeared at many locations in the nuclear genome of this plant, and contained a 21-bp element sharing 70% homology with the hypothetical replication origins of three viruses of Nicotiana spp. Two copies of this 21-bp element are overlapped with a 10-residue palindromic sequence. The possible biological roles of this repeated sequence and of the DNA-binding nuclear factor(s) are discussed. The application of this repeated sequence in the study of this plant genome is proposed.

• 出版日期1993-10
• 单位中国科学院植物研究所