Detection of renal brush border membrane enzymes for evaluation of renal injury in neonatal scleredema

作者：Ren, Ging; Zhang, Yongjun; Yang, Jinying; Wei, Lixia; Zhao, Lili; Yang, Giaozhi^*

来源：Pakistan Journal of Medical Sciences, 2015, 31(1): 65-69.

DOI：10.12669/pjms.311.5740

• beta 2-microglobulin
• Ligustrazine
• Neonatal scleredema
• Renal brush border membrane enzyme
• Renal injury

Objective: To evaluate renal brush border membrane enzymes in urine as an indicator for renal injury in neonatal scleredema(NS).
Methods: Sixty nine NS patients in our hospital were enrolled and divided into mild group and moderate/ severe group. Patients were further randomly divided into therapy and control subgroups for 7 days ligustrazine administration. Urine samples were collected to detect renal brush border membrane enzymes (RBBME) by ELISA and beta 2-microglobulin (beta(2)-MG) by radioimmunoassay (RIA). The results were compared, with those of 30 normal neonates. Data were statistically analyzed using SPSS13.0 software.,
Results: Both RBBME and beta(2)-MG were found to be higher in urine in NS patients than normal controls (P < 0.01). Level of RBBME increased with the severity of NS (P <0.05), while urinary beta(2)-MG did not (P >0.05). After being treated with ligustrazine, a medicine for renal function recovery, both RBBME and beta(1)-MG were. similarly significantly decreased comparing to untreated groups (P < 0.05). 79.7% of NS patients showed abnormal RBBME while only 52.2% had an abnormal urinary beta(2)-MG (x2=11.65, P < 0.01).
Conclusion: RBBME was more sensitive than 132-MG in reflecting the renal injury in NS. Examination of RBBME effectively reflected the recovery of renal injury after treatment with ligustrazine.

• 出版日期2015-2
• 单位聊城市人民医院