Azoic color reaction of flavonoids and their analytical application

作者：Yu, Q^*; Chen, XL; Luo, ZM; Yang, C; Mai, HK

来源：Spectroscopy and Spectral Analysis, 2004, 24(8): 991-994.

• flavonoid
• azoic color reaction
• spectrophotometry
• sophora seed
• soybean cake

In NH4 Cl-NH3 . H2O buffer solution of, pH 10.4, diazo salt of p-sulfodiazoben-zene chloride reacts with rutin, morin, genistin, quercetin, hesperidin, baicalin and daidzin to form color azo-compounds. These azo-compounds have an absorption maximum at 430 nm except for baicalin azo-compound, which has an absorption maximum at 380 nm. The sensitivity of azoic color reaction is reductive in the order of above flavonoids and the molar absorptivity is maximum for rutin and is minimal for daidzin among these azo-compounds, i.e. 3.28 x 10(4) and 8.3 x 10(2) L.mol(-1) . cm(-1) respectively. The proposed spectrophotometry has been applied to the determination of flavonoids in sophora seed and soybean cake. The RSDs of the method were found to be in the range of 0.75%-4.48%, and the recoveries were in the range of 99.3%-105.0%.

• 出版日期2004-8
• 单位广东工业大学; 韶关学院