MR Targeted Imaging for the Expression of Tenascin-C in Myocardial Infarction In Vivo

作者：Song, Jiacheng; Yu, Jing; Li, Yan; Lu, Shanshan; Ma, Zhanlong^*; Shi, Haibin

来源：Journal of Magnetic Resonance Imaging, 2017, 45(6): 1668-1674.

DOI：10.1002/jmri.25543

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PurposeTo investigate the presence of viable myocardium in mice with acute myocardial infarction (MI) using a molecular targeted probe. @@@ Materials and MethodsSuper paramagnetic iron oxide (SPIO) nanoparticles and tenascin-C antibody were conjugated as an MRI probe. Fifteen mice with infarction were injected with SPIO-anti-tenascin-C (3 days [d], 5d, 7d after infarction; n=5 for each group). Another five mice with infarction (5d, n=5) were injected with SPIO for comparison. In vivo MR (7 Tesla, fast low-angle shot multi-slice T2* sequence) was performed for tracing. Histological analysis was used to compare surviving cardiomyocytes with signal changes on MR. @@@ ResultsThe mRNA expression of tenascin-C increased directly after MI and peaked at the fifth day (5d 24.29 +/- 1.41 versus 3d 10.63 +/- 0.72, 7d 6.56 +/- 0.12; P<0.01). T-2 relaxation rate of synthesized SPIO-anti-tenascin-C was r2=338mM(-1)s(-1). After MR, the signal changes (contrast-to-noise ratio) of the research group were 3d 6.511.13 versus 5d 14.06 +/- 3.19 versus 7d 5.02 +/- 2.65, P<0.05. The MR signal showed a small decrease in the contrast group on 5d (research group 14.06 +/- 3.19 versus contrast group 1.75 +/- 0.59, P<0.05). @@@ ConclusionTenascin-C was expressed by surviving cardiomyocytes within the infarcted region. MR imaging with SPIO-anti-tenascin-C might be used to evaluate myocardial viability of MI patients before therapy.

• 出版日期2017-6
• 单位南京医科大学