A versatile assay for monitoring in vivo-like transendothelial migration of neutrophils

作者:Han Sewoon; Yan Ji Jing; Shin Yoojin; Jeon Jessie S; Won Jihee; Jeong Hyo Eun; Kamm Roger D; Kim Young Joon*; Chung Seok
来源:Lab on a Chip, 2012, 12(20): 3861-3865.
DOI:10.1039/c2lc40445a

摘要

Spatiotemporal analysis of the inflammatory response has been limited by the difficulties of in vivo imaging and reconstitution of inflammation in vitro. Here, we present a novel method for establishing in vivo-like inflammatory models in a microfluidic device and quantitatively measuring the three-dimensional transmigration of neutrophils during the inflammatory process. This enabled us to concurrently characterize transendothelial migration behaviors of neutrophils under the influence of various inflammatory stimuli.

  • 出版日期2012