The molecular basis of incomplete dominance at the A locus of CHS-D in the common morning glory, Ipomoea purpurea

作者：Johzuka Hisatomi Yasuyo^*; Noguchi Hiroshi; Iida Shigeru

来源：Journal of Plant Research, 2011, 124(2): 299-304.

DOI：10.1007/s10265-010-0369-7

• Anthocyanin pigmentation
• Chalcone synthase gene
• Dosage-dependent gene expression
• Flower variegation
• Incomplete dominance
• Ipomoea purpurea

The mutable a(flaked) (a(f)) allele at the A locus of the common morning glory (Ipomoea purpurea) confers incomplete dominance in flower pigmentation and is caused by insertion of the DNA transposon Tip100 into CHS-D, which encodes chalcone synthase and is required for anthocyanin biosynthesis. Levels of CHS-D transcripts, CHS-D protein, and anthocyanin pigment in heterozygous flowers were about half that in homozygous flowers, indicating that dosage-dependent expression of CHS-D is the primary cause of the observed incomplete dominance. This contrasts with the Nivea locus in snapdragon (Antirrhinum majus) in which incomplete dominance is caused by semidominant CHS alleles.

• 出版日期2011-3