Nicotine inhibits CD24 expression in Lewis lung carcinoma cells by upregulation of RAS expression

作者：Liu, Da-Hua; An, Min; Bao, Bai-Li; Ren, Fu^*; Xia, Pu^*

来源：International Journal of Oncology, 2018, 53(2): 815-822.

DOI：10.3892/ijo.2018.4415

• cluster of ddifferentiation 24
• lung cancer
• cancer stem cell
• nicotine
• prognosis

Cluster of ddifferentiation 24 (CD24) is a widely used cancer stem cell (CSC) marker in numerous cancer types. However, a number of studies have shown that CD24 is a prognostic marker, but not a CSC marker for lung adenocarcinoma. In the present study, firstly, bioinformatic analyses were used to identify the CD24 mRNA levels in the subtypes of lung cancer. Secondly, CD24(high) and CD24(low) cells were isolated from the side population of Lewis lung carcinoma (LLC) cells using flow cytometry. Furthermore, the stemness of CD24(high) and CD24(low) cells were determined in vivo and in vitro. Lastly, the mechanism(s) of nicotine-inhibited CD24 expression in LLC cells were assessed. The main findings of this study are that: i) CD24 could be used as a prognostic marker for human lung adenocarcinoma; ii) the in vitro and in vivo experiments did not determine a significant influence of CD24 on the tumorgenicity of LLC cells; and iii) nicotine inhibited CD24 expression in LLC cells by upregulation of RAS. However, the detailed mechanism(s) of these results require further analysis.

• 出版日期2018-8
• 单位锦州医科大学