A Simplified Positive-Sense-RNA Virus Construction Approach That Enhances Analysis Throughput

作者:Siridechadilok Bunpote*; Gomutsukhavadee Methee; Sawaengpol Thunyarat; Sangiambut Sutha; Puttikhunt Chunya; Chin inmanu Kwanrutai; Suriyaphol Prapat; Malasit Prida; Screaton Gavin; Mongkolsapaya Juthathip
来源:Journal of Virology, 2013, 87(23): 12667-12674.
DOI:10.1128/JVI.02261-13

摘要

Here we present an approach that advances the throughput of a genetic analysis of a positive-sense RNA virus by simplifying virus construction. It enabled comprehensive dissection of a complex, multigene phenotype through rapid derivation of a large number of chimeric viruses and construction of a mutant library directly from a virus pool. The versatility of the approach described here expands the applicability of diverse genetic approaches to study these viruses.

  • 出版日期2013-12