Analog Noise Reduction in Enzymatic Logic Gates

作者：Melnikov Dmitriy; Strack Guinevere; Pita Marcos; Privman Vladimir^*; Katz Evgeny

来源：Journal of Physical Chemistry B, 2009, 113(30): 10472-10479.

DOI：10.1021/jp904585x

In this work, we demonstrate both experimentally and theoretically that the analog noise generation by a single enzymatic logic gate can be dramatically reduced to yield gate operation with virtually no input noise amplification. We demonstrate that when a cosubstrate with a much smaller affinity than the primary substrate is used, a negligible increase in the noise output from the logic gate is obtained, as compared to the input noise level. OUT general theoretical conclusions were confirmed by experimental realizations of the AND logic gate based on the enzyme horseradish peroxidase using hydrogen peroxide as the substrate, with 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) or ferrocyanide as cosubstrates with vastly different rate constants.

• 出版日期2009-7-30