科研之友
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摘要
Gliomas are the most common type of malignant brain tumor. Studies have identified that miR-15b is negatively correlated with cripto-1 expression in glioma cells, and these molecules serve an important role in cancer development and progression. The current study was undertaken to further examine the association between these two molecules. Fluorescent quantitative PCR confirmed that miR-15b expression was significantly downregulated in glioma tissue while cripto-1 expression was significantly increased. Subsequent to transfection with miR-15b mimics, cripto-1 expression was significantly suppressed, and dual luciferase reporter assays further demonstrated that miR-15b regulates cripto-1 in a targeted manner. Furthermore, miR-15b inhibited proliferation and invasion, and promoted apoptosis of glioma cells while downregulating the expression of MMP-2 and MMP-9. In contrast, cripto-1 expression had the opposite effects. Co-transfection with miR-15b mimics and the cripto-1 overexpression vector overcame the inhibitory action of miR-15b on cripto-1. Therefore, it is suggested that miR-15b modulates cell growth and invasion through targeted regulation of cripto-1 expression in glioma cells. This observation may provide novel targets for the prevention and treatment of gliomas.
