Analysis of the expression and localization of tight junction transmembrane proteins, claudin-1,-4,-7, occludin and JAM-A, in human cervical adenocarcinoma

作者：Akimoto Taishi; Takasawa Akira^*; Murata Masaki; Kojima Yui; Takasawa Kumi; Nojima Masanori; Aoyama Tomoyuki; Hiratsuka Yutaro; Ono Yusuke; Tanaka Satoshi; Osanai Makoto; Hasegawa Tadashi; Saito Tsuyoshi; Sawada Norimasa

来源：Histology and Histopathology, 2016, 31(8): 921-931.

DOI：10.14670/HH-11-729

• Tight junction transmembrane protein
• Claudin
• JAM-A
• Immunohistochemistry
• Cervical adenocarcinoma
• Molecular marker

Objective. Tight junction proteins have recently been reported to be useful for distinguishing between neoplastic and non-neoplastic tissues. In this study, we evaluated the expression and localization of tight junction transmembrane proteins in human cervical adenocarcinoma and adenocarcinoma in situ (AIS), and we determined whether their expression patterns could distinguish cervical adenocarcinoma from nonneoplastic cervical glands. Methods. Fifty-five patients with cervical adenocarcinoma or AIS were included in this study. Surgical specimens were immunohistochemically stained for claudin (CLDN) -1, -4, -7, occludin, and JAM-A. Results. Significantly higher expression levels of CLDNs and JAM-A were found in cervical AIS and adenocarcinoma than in non-neoplastic glands. In cervical AIS and adenocarcinoma, localization of CLDN-1 and JAM-A was extended throughout the whole cell membranes, whereas they were predominantly expressed at the most apical cell-cell junction in non-neoplastic glands. ROC curve analysis revealed that immunoreactivities of CLDN-1 or JAM-A successfully distinguished neoplasms from nonneoplastic cervical glands with high specificity (CLDN-1, 79.1%; JAM-A, 79.1%) and high sensitivity (CLDN-1, 84.1%; JAM-A, 95.5%). Conclusions. As expected, there were immunohistochemical differences between cervical adenocarcinoma and non-neoplastic cervical glands by using antibodies against tight junction transmembrane proteins. These results suggest that CLDN-1 and JAM-A are potential biomarkers for cervical adenocarcinoma.

• 出版日期2016-8
• 单位河北医科大学