MicroRNA-128 suppresses colorectal cancer cell proliferation through targeting LRP6

作者：He, Dedong^*; Bai, Yu; Zhang, Dezhong; Fang, Xiangjie; Yang, Qinghui

来源：International Journal of Clinical and Experimental Pathology, 2016, 9(4): 4351-4359.

• miR-128
• colorectal cancer
• proliferation
• LRP6

Background: Colorectal cancer (CRC) is one of the most commonly cancer worldwide and many studies demonstrated that microRNAs (miRNAs) play important roles in the tumorigenesis and development of CRC. The purpose of this study was to investigate the expression level and biological functions of miR-128 in CRC. Methods: The expression level of miR-128 in 30 CRC specimens and 5 CRC cell lines was detected by quantitative real-time PCR (qRT-PCR). Cell proliferation was assessed by CCK-8 assay. Flow cytometry was performed to analyze the cell apoptosis and cell cycle distribution. Moreover, luciferase reporter assay was conducted to explore the potential target of miR-128 in CRC cells. Results: MiR-128 was significantly down-regulated in CRC specimens as well as in CRC cell lines. Overexpression of miR-128 in SW480 cells transfected with miR-128 mimics could suppress CRC cell proliferation and induces cell cycle arrest and cell apoptosis. In addition, we found that LRP6 was a direct and functional target of miR-128. In keeping with the effect induced by overexpression of miR-128, knockdown of LRP6 inhibited CRC cell proliferation. Conclusion: Our study indicated that miR-128 could suppress CRC cell proliferation through targeting LRP6. MiR-128 might act as a potential therapeutic target for CRC treatment in the future.

• 出版日期2016
• 单位新乡医学院