A carbon nanotube-polymer composite for T-cell therapy

作者：Fadel Tarek R^*; Sharp Fiona A; Vudattu Nalini; Ragheb Ragy; Garyu Justin; Kim Dongin; Hong Enping; Li Nan; Haller Gary L; Pfefferle Lisa D; Justesen Sune; Herold Kevan C; Fahmy Tarek M

来源：Nature Nanotechnology, 2014, 9(8): 639-647.

DOI：10.1038/NNANO.2014.154

Clinical translation of cell therapies requires strategies that can manufacture cells efficiently and economically. One promising way to reproducibly expand T cells for cancer therapy is by attaching the stimuli for T cells onto artificial substrates with high surface area. Here, we show that a carbon nanotube-polymer composite can act as an artificial antigen-presenting cell to efficiently expand the number of T cells isolated from mice. We attach antigens onto bundled carbon nanotubes and combined this complex with polymer nanoparticles containing magnetite and the T-cell growth factor interleukin-2 (IL-2). The number of T cells obtained was comparable to clinical standards using a thousand-fold less soluble IL-2. T cells obtained from this expansion were able to delay tumour growth in a murine model for melanoma. Our results show that this composite is a useful platform for generating large numbers of cytotoxic T cells for cancer immunotherapy.

• 出版日期2014-8