A sensitive assay for simultaneous determination of plasma concentrations of valganciclovir and its active metabolite ganciclovir by LC/MS/MS

作者：Xu, Hong-Rong^*; Li, Xue-Ning; Chen, Wei-Li; Liu, Gang-Yi; Chu, Nan-Nan; Yu, Chen

来源：Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2007, 848(2): 329-334.

DOI：10.1016/j.jchromb.2006.10.053

• valganciclovir
• ganciclovir
• aciclovir
• LC/MS/MS
• protein precipitation

A protein precipitation, liquid chromatography/tandem mass spectrometry (LC/MS/MS) method has been developed and validated for the simultaneous determination of valganciclovir and its active metabolite ganciclovir in human plasma. The solvent system also served as a protein precipitation reagent. The chromatographic separation was achieved on an Aquasil C18 column (50mm x 2.1 mm, 5 mu m). A linear gradient mobile phase between 0.02% formic acid and methanol was used. Detection was by positive ion electrospray tandem mass spectrometry on a Sciex API3000. The standard curves, which ranged from 4 to 512 ng/mL for valganciclovir and from 0.1 to 12.8 mu g/mL for ganciclovir, were fitted to a 1/x weighted quadratic regression model. The method was proved to be accurate, specific and sensitive enough and was successfully applied to a pharmacokinetic study.

• 出版日期2007-4-1
• 单位复旦大学; 上海市徐汇区中心医院