A far-red emitting fluorescent marker protein, mGarnet2, for microscopy and STED nanoscopy

作者：Matela Gabriela; Gao Peng; Guigas Gernot; Eckert Antonia F; Nienhaus Karin; Nienhaus G Ulrich^*

来源：Chemical communications, 2017, 53(5): 979-982.

DOI：10.1039/c6cc09081h

Here we presentmGarnet2, amonomeric, far-red fluorescentmarker protein derived from mRuby, with absorption and emission bands peaking at 598 and 671 nm, respectively. The protein shows excellent performance as a live-cell fusion marker for STED nanoscopy with 640 nm excitation and 780 nm depletion wavelengths.

• 出版日期2017