alpha-Amylase Monitoring by a Novel Amperometric Biosensor Based on Au Electrode: Its Optimization, Characterization, and Application

作者：Mengulluoglu Umut^*; Altug Cagri; Ertugrul H Deniz; Yildiz Abdulkerim; Ekici E Melis; Dinckaya Erhan

来源：Artificial Cells, Blood Substitutes, and Biotechnology, 2012, 40(1-2): 97-102.

DOI：10.3109/10731199.2011.597757

• alpha-Amylase
• Activity determination
• Glucose oxidase biosensor
• Amperometric biosensor
• Au electrode

A low-cost and sensitive amperometric biosensor was developed for the determination of alpha-amylase activity. The biosensor was constructed by immobilizing glucose oxidase-gelatin via glutaraldehyde on the Au electrode surface. Measurements were carried out chronoamperometrically at -0.7 V. Several parameters such as glucose oxidase activity, gelatin amount, and glutaraldehyde percentage for cross-linking were optimized. Optimum pH, optimum temperature, repeatability, and storage stabilities of the biosensor were identified. Under the optimum experimental conditions, a linear calibration curve was obtained for alpha-amylase between 0.819 and 13.110 U/ml. Sample analyses were carried out by detecting alpha-amylase activities in baker%26apos;s yeast samples.

• 出版日期2012-2