Multiple roles of PPAR alpha in brown adipose tissue under constitutive and cold conditions

作者：Komatsu, Makiko; Tong, Yuhong; Li, Yufeng; Nakajima, Takero; Li, Gang; Hu, Rui; Sugiyama, Eiko; Kamijo, Yuji; Tanaka, Naoki; Hara, Atsushi^*; Aoyama, Toshifumi

来源：Genes to Cells, 2010, 15(2): 91-100.

DOI：10.1111/j.1365-2443.2009.01368.x

• ACTIVATED RECEPTOR-ALPHA
• BETA-OXIDATION
• DEHYDROGENASE
• EXPRESSION
• LIVER
• GENE
• PROTEIN
• COACTIVATOR
• SUPPRESSION
• DEFICIENCY

Peroxisome proliferator-activated receptor alpha (PPAR alpha) is a member of the nuclear receptor family, regulating fatty acid degradation in many organs. Two-dimensional SDS-PAGE of brown adipose tissue (BAT) from PPAR alpha-null mice produced a higher-density spot. Proteomic analysis indicated that the protein was pyruvate dehydrogenase beta (PDH beta). To observe PDH beta regulation in BAT, the organ was stimulated by long-term cold exposure, and the activities of associated enzymes were investigated. Histological and biochemical analyses of BAT showed a significant decrease in the triglyceride content in wild-type mice and some degree of decrease in PPAR alpha-null mice on cold exposure. Analyses of molecules related to glucose metabolism showed that the expression of PDH beta is under PPAR alpha-specific regulation, and that glucose degradation ability may decrease on cold exposure. In contrast, analyses of molecules related to fatty acid metabolism showed that numerous PPAR alpha/gamma target molecules are induced on cold exposure, and that fatty acid degradation ability in wild-type mice is markedly enhanced and also increases to same degree in PPAR alpha-null mice on cold exposure. Thus, this study proposes novel and multiple roles of PPAR alpha in BAT.

• 出版日期2010-2
• 单位河北省人民医院; 河北医科大学