Size-dependent self-assembly of submicron/nano beads-protein conjugates for construction of a protein nanoarray

作者：Powell Tremaine B; Tran Phat L; Kim Keesung; Yoon Jeong Yeol^*

来源：Materials Science and Engineering: C , 2009, 29(8): 2459-2463.

DOI：10.1016/j.msec.2009.07.010

• Protein nanoarray
• Self-assembly
• Gold nanoparticles
• Fluorescent beads
• Electron beam lithography
• Fluorescent attenuation

A protein nanoarray is created when submicro and nano beads, varying in their size and each conjugated with different proteins, self-assemble to specific locations depending on the diameter matching the surface electron beam patterns created. Protein binding is confirmed from the fluorescence attenuation of the beads upon antigen-antibody binding on the bead surface. This method, called size-dependent self-assembly, allows control of the location of each type of bead, and thus, control of the location of multiple proteins. It provides fast multi-component patterning with a high binding resolution. which can be detected using a fluorescent light microscope. This method is developed to be a simple stand-alone tool for analysis of protein interactions. In addition, it has the potential to be used in conjunction with other protein analysis methods, such as enzyme-linked immunosorbent assay (ELISA) and atomic force microscopy (AFM).

• 出版日期2009-10-15