Hyaluronan inhibits BMP-induced osteoblast differentiation

作者：Kaneko Keiko; Higuchi Chikahisa; Kunugiza Yasuo; Yoshida Kiyoshi; Sakai Takashi; Yoshikawa Hideki; Nakata Ken^*

来源：FEBS LETTERS, 2015, 589(4): 447-454.

DOI：10.1016/j.febslet.2014.12.031

• Hyaluronan
• CD44
• Osteoblast
• Bone morphogenetic protein

Hyaluronan (HA), one of the major structural extracellular components in cartilage, regulates cellular responses via receptors such as CD44. However, the direct effects of HA on osteoblastic differentiation has not been studied in detail. Here, we investigated the effects of HA (molecular weight: 900-1200kDa) on osteoblastic differentiation that was induced by bone morphogenetic protein (BMP) in C2C12 cells (mouse myoblastic cells) and ST2 cells (mouse bone marrow cells). BMPinduced osteoblastic differentiation and Smad1/Smad5/Smad8 phosphorylation were downregulated by HA. Use of the CD44-blocking antibody restored HA-induced inhibition of osteoblastic differentiation and Smad1/Smad5/Smad8 phosphorylation. Our results indicate that HA inhibits BMPinduced osteoblastic differentiation through the CD44 receptor.

• 出版日期2015-2-13