A Validated HPLC Method for the Determination of GABA by Pre-Column Derivatization with 2,4-Dinitrofluorodinitrobenzene and Its Application to Plant GAD Activity Study

作者：Lue, Yingguo; Zhang, Hui^*; Meng, Xiangyong; Wang, Li; Guo, Xiaona

来源：Analytical Letters, 2010, 43(17): 2663-2671.

DOI：10.1080/00032711003763558

• -aminobutyric acid
• 2
• 4-dinitrofluorodinitrobenzene
• Glutamate decarboxylase
• HPLC
• Pre-column derivatization reaction

A validated, selective, and sensitive chromatographic method for determination of -aminobutyric acid (GABA) has been developed by precolumn derivative of the sample with 2,4-dinitrofluorodinitrobenzene (FDNB). The derivatives were separated with RP-HPLC on a C18 column and UV detection (360nm). The calibration curve was linear over a range of 0.2-5.0mg/mL GABA with a correlation coefficient of 0.9954. The precision of this method is high (RSD=0.061%). This method also possessed high recovery rate (99%) and good stability over a period of four weeks. The method was successfully used to determine the glutamate decarboxylase (GAD) activity in several plant tissues.

• 出版日期2010
• 单位河南工业大学; 江南大学