mTORC1-dependent AMD1 regulation sustains polyamine metabolism in prostate cancer

作者：Zabala Letona Amaia; Arruabarrena Aristorena Amaia; Martin Martin Natalia; Fernandez Ruiz Sonia; Sutherland James D; Clasquin Michelle; Tomas Cortazar Julen; Jimenez Jose; Torres Ines; Phong Quang; Ximenez Embun Pilar; Bago Ruzica; Galde Olano Aitziber U; Oizaga Iriarte Ana L; Acasa Viscasillas Isabel L; Nda Miguel U; Torrano Veronica; Cabrera Diana; van Liempd Sebastiaan M; Cendon Ylenia; Castro Elena; Murray Stuart; Revandkar Ajinkya; Alimonti Andrea; Zhang Yinan

来源：Nature, 2017, 547(7661): 109-+.

DOI：10.1038/nature22964

Activation of the PTEN-PI3K-mTORC1 pathway consolidates metabolic programs that sustain cancer cell growth and proliferation(1,2). Here we show that mechanistic target of rapamycin complex 1 (mTORC1) regulates polyamine dynamics, a metabolic route that is essential for oncogenicity. By using integrative metabolomics in a mouse model(3) and human biopsies(4) of prostate cancer, we identify alterations in tumours affecting the production of decarboxylated S-adenosylmethionine (dcSAM) and polyamine synthesis. Mechanistically, this metabolic rewiring stems from mTORC1-dependent regulation of S-adenosylmethionine decarboxylase 1 (AMD1) stability. This novel molecular regulation is validated in mouse and human cancer specimens. AMD1 is upregulated in human prostate cancer with activated mTORC1. Conversely, samples from a clinical trial with the mTORC1 inhibitor everolimus(5) exhibit a predominant decrease in AMD1 immunoreactivity that is associated with a decrease in proliferation, in line with the requirement of dcSAM production for oncogenicity. These findings provide fundamental information about the complex regulatory landscape controlled by mTORC1 to integrate and translate growth signals into an oncogenic metabolic program.

• 出版日期2017-7-6